Naringenin restricts the colonization and growth of Ralstonia solanacearum in tobacco mutant KCB-1.

Bacterial wilt severely jeopardizes plant growth and causes enormous economic loss in the production of many crops, including tobacco (Nicotiana tabacum). Here, we first demonstrated that the roots of bacterial wilt-resistant tobacco mutant KCB-1 can limit the growth and reproduction of Ralstonia solanacearum. Secondly, we demonstrated that KCB-1 specifically induced an upregulation of naringenin content in root metabolites and root secretions. Further experiments showed that naringenin can disrupt the structure of R. solanacearum, inhibit the growth and reproduction of R. solanacearum, and exert a controlling effect on bacterial wilt. Exogenous naringenin application activated the resistance response in tobacco by inducing the burst of reactive oxygen species and salicylic acid deposition, leading to transcriptional reprogramming in tobacco roots. Additionally, both external application of naringenin in CB-1 and overexpression of the Nicotiana tabacum chalcone isomerase (NtCHI) gene, which regulates naringenin biosynthesis, in CB-1 resulted in a higher complexity of their inter-root bacterial communities than in untreated CB-1. Further analysis showed that naringenin could be used as a marker for resistant tobacco. The present study provides a reference for analyzing the resistance mechanism of bacterial wilt-resistant tobacco and controlling tobacco bacterial wilt.

Machine Learning Prediction of Quantum Yields and Wavelengths of Aggregation-Induced Emission Molecules.

The aggregation-induced emission (AIE) effect exhibits a significant influence on the development of luminescent materials and has made remarkable progress over the past decades. The advancement of high-performance AIE materials requires fast and accurate predictions of their photophysical properties, which is impeded by the inherent limitations of quantum chemical calculations. In this work, we present an accurate machine learning approach for the fast predictions of quantum yields and wavelengths to screen out AIE molecules. A database of about 563 organic luminescent molecules with quantum yields and wavelengths in the monomeric/aggregated states was established. Individual/combined molecular fingerprints were selected and compared elaborately to attain appropriate molecular descriptors. Different machine learning algorithms combined with favorable molecular fingerprints were further screened to achieve more accurate prediction models. The simulation results indicate that combined molecular fingerprints yield more accurate predictions in the aggregated states, and random forest and gradient boosting regression algorithms show the best predictions in quantum yields and wavelengths, respectively. Given the successful applications of machine learning in quantum yields and wavelengths, it is reasonable to anticipate that machine learning can serve as a complementary strategy to traditional experimental/theoretical methods in the investigation of aggregation-induced luminescent molecules to facilitate the discovery of luminescent materials.

Combined control algorithm based on synchronous reinforcement learning for a self-balancing bicycle robot.

In this paper, balance control of a bicycle robot is studied without either a trail or a mechanical regulator when the robot moves in an approximately rectilinear motion. Based on the principle of moment balance, an input nonaffine nonlinear dynamics model of the bicycle robot is established. A driving velocity condition is proposed to maintain the robot balance. The nonaffine nonlinear system is transformed into an affine nonlinear system by defining the equivalent control. Subsequently, a feedback linearization controller is designed for the equivalent control. We design a combined control algorithm of synchronous policy iteration based on the actor-critic architecture. The actor neural network (NN) is designed based on the feedback linearization control law. Weight tuning laws for the critic and actor NNs are proposed. The system closed-loop stability and convergence of the NN weights are guaranteed based on the Lyapunov analysis. The optimality of the equivalent control policy is guaranteed. To satisfy the driving velocity condition, the values of the steering angle and driving velocity are determined based on the optimal equivalent control. The effectiveness of the proposed algorithm is verified through simulations and real experiments.

Breast cancer detection and classification in mammogram using a three-stage deep learning framework based on PAA algorithm.

In recent years, deep learning has been used to develop an automatic breast cancer detection and classification tool to assist doctors. In this paper, we proposed a three-stage deep learning framework based on an anchor-free object detection algorithm, named the Probabilistic Anchor Assignment (PAA) to improve diagnosis performance by automatically detecting breast lesions (i.e., mass and calcification) and further classifying mammograms into benign or malignant. Firstly, a single-stage PAA-based detector roundly finds suspicious breast lesions in mammogram. Secondly, we designed a two-branch ROI detector to further classify and regress these lesions that aim to reduce the number of false positives. Besides, in this stage, we introduced a threshold-adaptive post-processing algorithm with dense breast information. Finally, the benign or malignant lesions would be classified by an ROI classifier which combines local-ROI features and global-image features. In addition, considering the strong correlation between the task of detection head of PAA and the task of whole mammogram classification, we added an image classifier that utilizes the same global-image features to perform image classification. The image classifier and the ROI classifier jointly guide to enhance the feature extraction ability and further improve the performance of classification. We integrated three public datasets of mammograms (CBIS-DDSM, INbreast, MIAS) to train and test our model and compared our framework with recent state-of-the-art methods. The results show that our proposed method can improve the diagnostic efficiency of radiologists by automatically detecting and classifying breast lesions and classifying benign and malignant mammograms.

SALIS transcriptionally represses IGFBP3/Caspase-7-mediated apoptosis by associating with STAT5A to promote hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is the most common subtype of liver cancer and the second most fatal cancer in the world despite the great therapeutic advances in the past two decades, which reminds us of the gap in fully understanding the oncogenic mechanism of HCC. To explore the key factors contributing to the progression of HCC, we identified a LncRNA, termed SALIS (Suppression of Apoptosis by LINC01186 Interacting with STAT5A), functions in promoting the proliferation, colony formation, migration and invasion while suppressing apoptosis in HCC cells. Mechanistic study indicated SALIS physically associates with transcription factor STAT5A and binds to the promoter regions of IGFBP3 and Caspase-7 to transcriptionally repress their expression and further inhibit apoptosis. Our findings identified SALIS as an oncogene to promote HCC by physically binding with STAT5A to inhibit the expression of pro-apoptotic IGFBP3 and Caspase-7, which suggests novel therapeutic targets for HCC treatments.

Analysis and Evaluation of the Image Preprocessing Process of a Six-Band Multispectral Camera Mounted on an Unmanned Aerial Vehicle for Winter Wheat Monitoring.

Unmanned aerial vehicle (UAV)-based multispectral sensors have great potential in crop monitoring due to their high flexibility, high spatial resolution, and ease of operation. Image preprocessing, however, is a prerequisite to make full use of the acquired high-quality data in practical applications. Most crop monitoring studies have focused on specific procedures or applications, and there has been little attempt to examine the accuracy of the data preprocessing steps. This study focuses on the preprocessing process of a six-band multispectral camera (Mini-MCA6) mounted on UAVs. First, we have quantified and analyzed the components of sensor error, including noise, vignetting, and lens distortion. Next, different methods of spectral band registration and radiometric correction were evaluated. Then, an appropriate image preprocessing process was proposed. Finally, the applicability and potential for crop monitoring were assessed in terms of accuracy by measurement of the leaf area index (LAI) and the leaf biomass inversion under variable growth conditions during five critical growth stages of winter wheat. The results show that noise and vignetting could be effectively removed via use of correction coefficients in image processing. The widely used Brown model was suitable for lens distortion correction of a Mini-MCA6. Band registration based on ground control points (GCPs) (Root-Mean-Square Error, RMSE = 1.02 pixels) was superior to that using PixelWrench2 (PW2) software (RMSE = 1.82 pixels). For radiometric correction, the accuracy of the empirical linear correction (ELC) method was significantly higher than that of light intensity sensor correction (ILSC) method. The multispectral images that were processed using optimal correction methods were demonstrated to be reliable for estimating LAI and leaf biomass. This study provides a feasible and semi-automatic image preprocessing process for a UAV-based Mini-MCA6, which also serves as a reference for other array-type multispectral sensors. Moreover, the high-quality data generated in this study may stimulate increased interest in remote high-efficiency monitoring of crop growth status.

Deriving the Characteristic Scale for Effectively Monitoring Heavy Metal Stress in Rice by Assimilation of GF-1 Data with the WOFOST Model.

Accurate monitoring of heavy metal stress in crops is of great importance to assure agricultural productivity and food security, and remote sensing is an effective tool to address this problem. However, given that Earth observation instruments provide data at multiple scales, the choice of scale for use in such monitoring is challenging. This study focused on identifying the characteristic scale for effectively monitoring heavy metal stress in rice using the dry weight of roots (WRT) as the representative characteristic, which was obtained by assimilation of GF-1 data with the World Food Studies (WOFOST) model. We explored and quantified the effect of the important state variable LAI (leaf area index) at various spatial scales on the simulated rice WRT to find the critical scale for heavy metal stress monitoring using the statistical characteristics. Furthermore, a ratio analysis based on the varied heavy metal stress levels was conducted to identify the characteristic scale. Results indicated that the critical threshold for investigating the rice WRT in monitoring studies of heavy metal stress was larger than 64 m but smaller than 256 m. This finding represents a useful guideline for choosing the most appropriate imagery.